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KMID : 0357319760110010001
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Journal of the Korean Society for Microbiology
1976 Volume.11 No. 1 p.1 ~ p.11
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Cytological Changes Associated with the Exposure of Escherichia coli to Several Disinfectants: An Ultrastructural Study
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Abstract
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An electron microscopic study has been made of the effects of change of cell structure of Escherichia coli treated with several disinfectants.
The alterations observed as follows:
1. Nucleoid, cytoplasm which contain ribosome and cell wall appeared to be composed of a parallel triple-layered membrane can observed in control Escherichia coli.
2. T he nuclear .material was no longer demonstrable in its normal sites. The cytoplasm lost its granularity, became homogeneous and disruption of cell wall were observed by the treatment with 70% ethyl alcohol and 3% H´O´.
3. Aggregation of ribosome and condensation of nuclear material were also observed by the treatment of 5% Lysol solution and 1% dodecyl-diamino-ethyl-glycin-hydrochloride.
4. In autoclaved group, the each layer of cell wail was -separated and destroyed in some sites where cytoplasm was extracted.
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